Detaily projektu
Team Name: BBM mikrobiyoloji
Gökcan Göksoy
Cansu Akay
Věková skupina: 2 (15-20)
Země: Turkey
The Antioxidant and Antimicrobial Effect of Lamium galeobdolon L., Alpinia officinarum L. and Peganum harmala L.
Téma: Chemie potravin
Typ média: Presentation
Urinary system infections are the most common community acquired infections. The main reason of that is Escherichia coli (E. coli). People are referred to a doctor cause of urine infections every year around 8.3 millions in the world. On the other hand, active oxygen forms are formed during normal processes of body using oxygen in human metabolism. When these forms are not prevented, they would cause structural degradation of DNA, proteins, carbohydrates and lipids. Thus, they cause many degenerative diseases by disrupting the structure and function of the cell membrane. Synthetic antioxidants are effective and cheap for a long period of time, but their antioxidants have decreased their attractiveness when negative effects on human health are revealed. The World Health Organization (WHO) has reported that approximately 20,000 plants are used for medical treatments. For this purpose, it was aimed to investigate the antimicrobial and antioxidant effects of Lamium galeobdolon L., Alpinia officinarum L. and Peganum harmala L. plants which are used in public due to effects haven’t clarified yet.
The plants were prepared by extracting in methanol with Soxhlet extraction method. DPPH (2,2-diphenyl-1-picrylhydrazyl), which shows free radical properties for antioxidant activity determination, was dissolved in methanol and they were used at different concentrations. Free radical scavenger fractions of the extracts were calculated using spectrophotometer and the concentration / inhibition percentages were plotted. For the determination of antimicrobial activity, plant extracts were evaluated by using E. coli bacteria. Therefore cefotaxime antibiotic was used as a positive control and methanol was used as a negative control. Disc diffusion diameters were calculated according to the antibiotics of each plant extraction and compared with each control group.
In this respect, we have demonstrated free radical scavenging activities and antimicrobial activities by using Alpinia officinarum L., Peganum harmala L. and Lamium galeobdolon L. plants, which have been used by the public but whose effects haven’t enough researches yet. Finally by chosen these plants, we have shown that Alpinia officinarum L. can be used as a new natural resource in food and pharmaceutical fields due to its strong antimicrobial and antioxidant properties.
The plants were prepared by extracting in methanol with Soxhlet extraction method. DPPH (2,2-diphenyl-1-picrylhydrazyl), which shows free radical properties for antioxidant activity determination, was dissolved in methanol and they were used at different concentrations. Free radical scavenger fractions of the extracts were calculated using spectrophotometer and the concentration / inhibition percentages were plotted. For the determination of antimicrobial activity, plant extracts were evaluated by using E. coli bacteria. Therefore cefotaxime antibiotic was used as a positive control and methanol was used as a negative control. Disc diffusion diameters were calculated according to the antibiotics of each plant extraction and compared with each control group.
In this respect, we have demonstrated free radical scavenging activities and antimicrobial activities by using Alpinia officinarum L., Peganum harmala L. and Lamium galeobdolon L. plants, which have been used by the public but whose effects haven’t enough researches yet. Finally by chosen these plants, we have shown that Alpinia officinarum L. can be used as a new natural resource in food and pharmaceutical fields due to its strong antimicrobial and antioxidant properties.
.%20People%20are%20referred%20to%20a%20doctor%20cause%20of%20urine%20infections%20every%20year%20around%208.3%20millions%20in%20the%20world.%20On%20the%20other%20hand%2C%20active%20oxygen%20forms%20are%20formed%20during%20normal%20processes%20of%20body%20using%20oxygen%20in%20human%20metabolism.%20When%20these%20forms%20are%20not%20prevented%2C%20they%20would%20cause%20structural%20degradation%20of%20DNA%2C%20proteins%2C%20carbohydrates%20and%20lipids.%20Thus%2C%20they%20cause%20many%20degenerative%20diseases%20by%20disrupting%20the%20structure%20and%20function%20of%20the%20cell%20membrane.%20Synthetic%20antioxidants%20are%20effective%20and%20cheap%20for%20a%20long%20period%20of%20time%2C%20but%20their%20antioxidants%20have%20decreased%20their%20attractiveness%20when%20negative%20effects%20on%20human%20health%20are%20revealed.%20The%20World%20Health%20Organization%20(WHO)%20has%20reported%20that%20approximately%2020%2C000%20plants%20are%20used%20for%20medical%20treatments.%20For%20this%20purpose%2C%20it%20was%20aimed%20to%20investigate%20the%20antimicrobial%20and%20antioxidant%20effects%20of%20Lamium%20galeobdolon%20L.%2C%20%20Alpinia%20officinarum%20L.%20and%20Peganum%20harmala%20L.%20plants%20which%20are%20used%20in%20public%20due%20to%20effects%20haven%E2%80%99t%20clarified%20yet.%0D%0AThe%20plants%20were%20prepared%20by%20extracting%20in%20methanol%20with%20Soxhlet%20extraction%20method.%20DPPH%20(2%2C2-diphenyl-1-picrylhydrazyl)%2C%20which%20shows%20free%20radical%20properties%20for%20antioxidant%20activity%20determination%2C%20was%20dissolved%20in%20methanol%20and%20they%20were%20used%20at%20different%20concentrations.%20Free%20radical%20scavenger%20fractions%20of%20the%20extracts%20were%20calculated%20using%20spectrophotometer%20and%20the%20concentration%20%2F%20inhibition%20percentages%20were%20plotted.%20For%20the%20determination%20of%20antimicrobial%20activity%2C%20plant%20extracts%20were%20evaluated%20by%20using%20E.%20coli%20bacteria.%20Therefore%20cefotaxime%20antibiotic%20was%20used%20as%20a%20positive%20control%20and%20methanol%20was%20used%20as%20a%20negative%20control.%20Disc%20diffusion%20diameters%20were%20calculated%20according%20to%20the%20antibiotics%20of%20each%20plant%20extraction%20and%20compared%20with%20each%20control%20group.%20%0D%0AIn%20this%20respect%2C%20we%20have%20demonstrated%20free%20radical%20scavenging%20activities%20and%20antimicrobial%20activities%20by%20using%20Alpinia%20officinarum%20L.%2C%20Peganum%20harmala%20L.%20and%20Lamium%20galeobdolon%20L.%20plants%2C%20which%20have%20been%20used%20by%20the%20public%20but%20whose%20effects%20haven%E2%80%99t%20enough%20researches%20yet.%20Finally%20by%20chosen%20these%20plants%2C%20we%20have%20shown%20that%20Alpinia%20officinarum%20L.%20can%20be%20used%20as%20a%20new%20natural%20resource%20in%20food%20and%20pharmaceutical%20fields%20due%20to%20its%20strong%20antimicrobial%20and%20antioxidant%20properties.){kind=link}